Triple lumen Bio-Printer head

thingiverse

This prototype is merely an experiment, so don't expect perfection. The primary objective is to explore the potential of new technology and creative designs. For instance, this project's components cannot be created using injection molding or CNC milling; thus, additive manufacturing seems ideal. Attached are test prints demonstrating the feasibility of making Bio-Printer parts using a Form1 3D printer. These parts can adapt standard laboratory devices like syringes and butterfly needles to fit the Bio-Print head's channels. The process is surprisingly easy, fast, and accurate, with plans to coat inner channels with lipid. The prototype Bio-Printer features double and triple lumen feed heads with pipette, syringe, and tubing connectors. These components will be attached to a gantry controlled by EASYCNC-EZ DIY controller and MACH3 CAM software. Creating simple 3D shapes in CAD programs like TrueSpace generates G-Code for controlling Bio-print head movements using DeskProto. Future developments may include LABVIEW controlling syringe pumps or solenoid valves with a Phidget SSR board, possibly synchronizing print head movement with pump activation. Additionally, a mini-TopPrint idea involves a cutter head creating a rough shape before swapping in the bio-print head to distribute cells on contoured surfaces. Initial test prints may use poppy seeds and grow matrix, followed by short-term cell culture samples with various cell types and collagen or albumin. The Bio-printer's channels can be 100 microns wide, with an inner mixing chamber added if necessary, and a side viewport for a camera as well.