RAF kinase

thingiverse

This is one part of dimer of RAF protein kinase, and its entire structure contains a dimer of the RAF kinase domain at its center along with two copies of MEK attached on the outside. As we know, RAF plays crucial roles in cell metabolism, and if it has mutations in just one nucleotide within its gene, it will have severe consequences. Therefore, I want to show people exactly what this looks like. And if you want to verify the information for this molecule, you can check out the website at http://pdb101.rcsb.org/motm/195. Furthermore, if you want to download the STL file to make some modifications, you can do so from RCSB at http://www.rcsb.org/pdb/explore/explore.do?structureId=4mne. Printer Settings: Brand: MakerBot Printer: MakerBot Replicator (5th Generation) Rafts: No Supports: Yes Notes: This molecule was also designed in VMD software and modified in Tinkercad. When you convert the STL file to Tinkercad, be aware of its scale, which should be between 90-95%. The supports I used are 3.5mm in diameter. You should note that the minimum diameter for supports is 3.0mm. If you're concerned about flaws when printing, add more supports and rafts. Higher supports need to be reinforced. When I first finished this molecule, I was thrilled to see it because it looked beautiful and had some minor flaws. The supports are pretty strong mostly, but there were still issues with the supports as you can see from the pictures I posted. Therefore, I made some modifications to strengthen my supports. Post-Printing: From the pictures I posted, you can see that I haven't finished trimming and modifying because I don't have a knife in hand and need to do it manually. Therefore, if you can do it yourself, use a knife to make this process easier.