Protein Structure Cookie Cutter (PRC2-AEBP2)

Protein Structure Cookie Cutter (PRC2-AEBP2)

prusaprinters

<p>Cookie cutter in the shape of a negative stain electron microscopy-derived protein structure of the protein complex PRC2-AEBP2, an essential histone methyltransferase responsible for the maintenance of gene repression.</p> <p>I based the design on figures contained in the following paper. I chose this structure because it is in an open-access journal (no paywall), and because I thought it would look good as a cookie:</p> <p>Ciferri, C., Lander, G.C., Maiolica, A., Herzog, F., Aebersold, R. and Nogales, E., 2012. Molecular architecture of human polycomb repressive complex 2. elife, 1, p.e00005.</p> <p>The main alteration I made was to widen the bridging section that joins the upper and lower lobes of the complex to prevent the resulting cookies breaking in half too easily.</p> <p>If you don't have a recipe to start with, I've tested and included photos of cookies I've made using this recipe: <a href="https://youtu.be/hvFNVAOvOXk">https://youtu.be/hvFNVAOvOXk</a></p> <h3>Print Settings</h3> <p><strong>Rafts:</strong></p> <p>No</p> <p class="detail-setting supports"><strong>Supports: </strong> <div><p>No</p></div> <p><strong>Resolution:</strong></p> <p>0.2mm</p> <p class="detail-setting infill"><strong>Infill: </strong> <div><p>20 - 100%</p></div> <p><strong>Filament:</strong> 3D Fillies PLA, PETG <br/> <p class="detail-setting notes"><strong>Notes: </strong></p> </p><div><p>Use a foodsafe filament such as PLA or PETG (from manufacturers who do not add toxic additives, such as the PLA+ from 3D Fillies).</p> Use a food-grade stainless steel nozzle (such as the E3D Stainless Steel Nozzle V6), as many brass nozzle are soft and contain toxic additives. <p>Use at least 2 vertical perimeters and minimise infill layers (i.e. use solid layers) in order to increase the strength of the final product. This is important as you'll be pressing down on them quite hard when making cookies.</p> </div></p></p> Category: Kitchen & Dining

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