PCR Tube Cooler (for 1.5 ml and 100-200 microliter tubes)

thingiverse

Human: Laboratory PCR Tube Cooler for keeping your PCR reagents cool while you do your PCR prep. This accommodates 96 0.1-0.2 ml PCR tubes and 10 1.5ml tubes. It can also fit most PCR reagent vials (up to 10). INSTRUCTIONS FOR USE: Print the calibration piece and determine how much vertical shell offset you need for your tubes to fit (approximately -0.15 to -0.20 mm). Use the same settings in printing the rest of the Main Body and Tube Holder. Fill with shaved ice up to the second line before placing the Tube holder on top. You can also use plain water if you don't have an ice maker. Fill the Main Body with distilled water up to the first line, then freeze. Before using, fill it with distilled cold water up to the 2nd line before placing the Tube holder on top. The holder can keep your tubes cool between 30-60 minutes (approximate). Replenish ice as needed. I still need to adjust this to fit PCR tube strips, but in the meantime, it may only fit single tubes. Print Settings Printer Brand: RepRap Printer: Wanhao i3v2 Rafts: No Supports: No Resolution: 100-200 microns Infill: 50% Notes: Print at slower speeds with 3 outer shells (for 0.4mm nozzles) to ensure the Main body is water-tight. You may need to adjust your top shell density depending on the printer. Print with at least 1mm thick top shell. Post-Printing Test the Main Body if it is watertight. If not, seal with cyanoacrylate or clear gloss.