Nitrogenase-Cutaway

thingiverse

This is a detailed view of the nitrogenase enzyme complex, a critical system that nitrogen-fixing organisms utilize to convert atmospheric nitrogen (~78% of the atmosphere) into ammonia, an essential precursor for constructing proteins and DNA. It depicts the segment of the enzyme that houses the two unique metal clusters that play a vital role in this process. The included FeMo-cofactor features homocitrate and P-cluster alongside various surrounding amino acids. This is an exemplary illustration of how nature skillfully integrates intricate molecular clusters to create a potent catalyst. After completing the model, I added colors to the cofactors - sulfur yellow and iron green - along with secondary structure elements. The brackets were painted with steel paint to denote that they are not part of the actual structure. The model's framework is based on the 1MIN protein data bank crystal structure. Using VMD, I manipulated the structure before transferring it to Tinkercad for further refinement and support addition. This detailed model was created using a Makerbot Replicator 2 printer. I incorporated supports as well as included instructions. Additionally, I scaled up my design to 150% of the original size, which allowed for a slightly larger, more robust end result. However, it did take around 40 hours at medium quality print settings. In order to prepare the structure for viewing, I had to manually remove scaffolding and spent additional time refining details with a modeling knife before adding final colors and texturing. With caution advised due to the substantial print duration, I would recommend following these steps closely. Some additional images have been included for your reference. Note: I am working on multiple related projects currently, including additional detailed models of essential enzymes and an even larger initiative planned for this summer.