Microscope upgrade to Fluoresence

thingiverse

The 3D printed attachment for microscopy relies heavily on the fundamental principles of fluorescence microscopy. A built-in flashlight emits white light that passes through an excitation filter, which allows only a specific wavelength to pass. This filtered light is then reflected onto the sample, where it triggers emission, which in turn passes through the dichroic mirror and emission filter before being captured by a digital laboratory camera. The attachment features a compact design with a house and three customizable compartments containing cube-like structures that can be easily swapped for different fluorescence proteins. Each compartment has a chute for an excitation filter, dichroic mirror, and emission filter, which can be selected specifically to match the type of fluorescence protein being studied. Conjugaid has successfully employed this microscope attachment to identify GFP in their samples using Roscolux filters. For GFP detection, they chose specific filters - #85 for excitation and #389 for emission - based on the known fluorescence spectrum of GFP.