Microscope slide well plate adapter for live cell imaging

thingiverse

Live cell imaging chamber to keep cells contained in humidified and heated environment. Meant for microscopes that have large enclosures to keep temperatures constant. The PLA or PET-C printed chambers keep fluids in the adapter pretty well, wall thickness was increased to minimise leakages. We prefer to place wetted tissues in the adapter. The adapted consists of 4 parts: a base, a midpiece, a lid and a wedge and a 64.4x88.6 mm glass plate (we cut an old SDS page glass plate to fit). The base contains the basin to keep moisture (water or wetted tissues), the midpiece allows attaching a gas line to provide a proper CO2/O2 gas mixture, the lid can easily slide off to add components to the wells without disturbing the positioning of the sample. Note that the lid is not heated, when used in a table top incubator setting this will generate condense on the top window, recommended use is within a large stage incubator setting.