
MesoSPIM V5
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Researchers are now empowered to work with high-tech tissue clearing methods that have undergone significant refinement over the past decade, with numerous advanced techniques readily available at their fingertips. Light-sheet microscopes, with their exceptional ability to create crisp images from translucent tissues, have been especially useful for visualizing large cleared samples. This specialized equipment has received substantial technological and commercial updates recently. Still, despite this notable progress, the scientific community is falling short when it comes to having tools capable of offering detailed examinations of large specimens within minutes, at a level similar to an isotropic resolution map. In this context, we present the mesoSPIM initiative as a comprehensive resource, where users are guided on how to easily construct and operate specialized light-sheet microscopes for studying samples of centimeter-sized cleared tissues, including free access to essential documentation at: http://www.mesospim.org, with an added note for interested individuals to view the project's initial research draft posted on: https://www.biorxiv.org/content/10.1101/577122v1.full
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