Mason Jar Bioreactor

thingiverse

Mason Jar Bioreactor Create a low-cost bioreactor with everyday materials! For use with wide-mouth Mason jars. This is still a work in progress. Bioreactors are typically used when doing tissue engineering to grow the cells needed for seeding after decellularization of some type of organic matter, often fruits, organs, or muscles. I designed this bioreactor to be very low-cost and to be printed on a resin printer as an ideal environment inside the reactor. It can also be printed on a common FDM machine, but I would keep it in a flow hood. There is a place for a bearing in the middle of the lid that takes a 16mm bearing. This is for a stirrer if you want to use one, but it's not really necessary. You can print a cap for the middle if you won't be using it. The procedure can be found here: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097835#s2 I used a strawberry in my decellularization, but you can use many other things. Some other great reads: http://www.pellinglab.net/using-fruits-for-in-vitro-3d-cell-culture/ http://ossificatorium.blogspot.com/2013/10/biomaterial-processes-diy-tissue.html