Firefly Luciferase 3D Print Ready

thingiverse

The protein structure of the Luciferase enzyme from the Japanese Firefly (Luciola cruciata) is visualized in this crystal model, represented as PDB 2D1S. Bound tightly to its substrate, luciferin, appears clearly as CPK. When the Luciferase enzyme combines with luciferin, Mg-ATP and oxygen, an excited oxyluciferin molecule plus visible light is produced. This detailed model highlights a critical amino acid residue, Serine at position 286 in the protein sequence, shown as CPK for emphasis. The reason for this focus lies in its crucial role: Serine generates greenish-yellow light; however, when it's replaced by Asparagine, red light is emitted instead. Organisms' light-emitting reactions are classified as bioluminescence, drawing significant research interest due to their importance in medical applications. Directions: In Makerbot software, scale the model 175% or set it to the largest possible size. Print using scaffolding or rafts for added stability. After printing, use needle-nosed pliers to carefully remove scaffolding from the top and sides, working inward methodically. Keep large support beams and base intact as you remove the scaffolds; they provide vital support. This model is extremely fragile and requires several hours to clear away the scaffolding completely. Consider maximizing infill options to enhance print strength.