Dual Index PCR work-up rack

thingiverse

This rack makes it easy to accurately aliquot dual index primers from the NEBNext Multiplex Oligos for Illumina kit because it holds the oligo tubes in the right spacing (9mm center to center) for a multichannel pipette. The primers come in screw cap tubes that have an outer diameter of about 8.13mm, so they don't fit well in other standard racks. I assume that Illumina Dual Index primers come in the same tube, but I've never worked with them. If there is "filament junk" in the primer tube holes, the fit can be tight. I just cleaned out the holes well, but I might add an STL with slightly bigger holes when I get a chance. This rack is 12x10, so there are two extra rows. I just did this because the rack that I modded to make this came as 12x10, so it was easier. And sometimes it's nice to have two extra rows. We recommend using a PCR work-up rack such as the one mentioned to assist in properly combining the index primers during the PCR amplification step. Alternatively, 96-well deep well plates can be used and aligned against a PCR plate as shown in the diagram below. NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600) I really wanted to buy a TruSeq Index Plate Fixture from Illumina. It was recommended in the NEBNext Multiplex Oligos for Illumina manual, and seemed easier than trying to design something to print. The funny thing is that Illumina didn't want to sell it to me, no matter how many times I tried to call or email their customer support. So I gave up trying to give money to Illumina. Their loss, your gain!