CRISPR / CAS9 Restriction enzyme set

prusaprinters

Gene editing is an exciting new field of genetics. This is done with the CRISPER (Clustered regularly interspaced short palindromic repeats) and Cas sequence restriction enzymes. This is a remix of my DNA Restriction Enzymes set. (http://www.thingiverse.com/thing:1304176) For the sake of space I removed the space from the Phosphate groups, and condensed the CRISPER target sequence. The CRISPER 2 file has a large cutout in it. This will help you see the DNA sequence it is replacing, and it cuts the material and print time almost in half. You can use both pieces to show how CRISPER / Cas9 is used in gene editing. You can also expand this to talk about bioethics, cancer treatment and the human genome. Print instructionsUnassociated tags: #biologyProject, #Cas9, #CRISPER, #DNA, #enzyme, #geneEditing, #geneticPRoject, #MakerEdChallenge2, #RNA, #scienceProject Category: Biology Print Settings Printer Brand: LulzBot Printer: TAZ 5 Rafts: Doesn't Matter Supports: Doesn't Matter Resolution: .2 - .3 Infill: 5% Notes: Break all sharp edges. Print 2 to show how CIRSPER is a palindrome sequence. Print the Cas9 the same as the RNA pieces from my other manipulative set. Print the CRIPSER in whatever color you have in bulk. To print this so the letters stand out, print the first base layers in a dark color (about 1.5 mm high), and then switch to a lighter filament about half way through. This will produce highly visible lettering that may help visually impaired students. Like the other restriction enzymes, you can also use a permanent marker to highlight the lettering. Post-Printing Post Print Print with my DNA / RNA Manipulative set. See the Restriction enzyme set for additional instructions. How I Designed This Designed This was a remix from my DNA / RNA manipulative sets. This set is the target sequence for CRISPER. More info on the sequence can be found here https://en.wikipedia.org/wiki/CRISPR To fit this onto a students desk, I compressed the target sequence by 1/2 to fit within the build volume of most 3D printers. Designed and remixed in tinkerCAD. Custom Section PROJECT CRISPER This is a project to expand upon others I have designed for genetics, DNA and RNA. You can cover DNA, RNA and gene editing with this set. Objectives Students will learn how CRISPER and Cas sequence RNA is used to edit genes. Students will examine how to change DNA with CRISPER. Students will examine the bioethics of using such technology. Students will examine the CRISPER method compared to other restriction enzymes. Students will explore how restriction enzymes work. Audiences This is for AP Biology, College biology, genetics, or medical professionals such as genetic councilors. Subjects AP Biology, College Biology, Genetics, Bioethics, Medical school Standards AP biology, College biology standards can be met with this set. Lesson See DNA / RNA manipulative, Restriction enzymes set for previous lessons. After learning about DNA, RNA, and restriction enzymes you can do the following. Model the CRISPER / CAS sequence and how it is used to edit genes. Explore how it is used to edit genes. Explore the bioethics of editing human and animal genes. Explore the positive and negative impacts of gene editing. Model and demonstrate how CRISPER can be used to produce a new gene sequence. Demonstrate how to treat a specific disorder with CRISPER. Duration: 1 - 3 weeks depending on the scope of your class. Preparation Students will need a set of the CRISPER / Cas9 pieces, as well as a full DNA / RNA manipulative set. You may want the Restriction enzymes set as well. You can 3D print the CRISPER in any filament you have in bulk. As Cas9 is a RNA sequence I recommend printing it the same color as the RNA pieces. You will need recent articles on CRISPER / Cas9 and articles on the bioethics of gene editing. References I started with https://en.wikipedia.org/wiki/CRISPR for the target sequence. Other Cas sequences may come latter. Assessment Students should demonstrate with these pieces how to edit genes. Students should show the bioethics of this new technology. Students should show how gene editing happens. Future lesson plans and activities will be posted on my teachersPayTeachers website.