Brain Slice Holder

thingiverse

For electrophysiology experiments on brain sections, a specialized chamber is required that features a mesh bottom capable of holding the brain sections securely in place. This chamber can be submerged into a glass beaker filled with a physiological solution carefully designed to maintain the viability of the brain slices. This design consists of two distinct components: The first part encompasses the chamber itself, while the second part comprises a table or stand for housing the chamber. Both parts can be connected using button magnets that will fit seamlessly into the four wells on both components. A tube providing oxygen can then be wrapped around for optimal aeration purposes. The decision to create two separate parts is driven by the need for the brain slices to rest on a mesh at the bottom of the chamber, necessitating that the chamber be printed and a mesh be attached using superglue at its base. Subsequently, the table can be secured in place using magnets. Photos will be uploaded in the near future once a copy has been produced in PLA and acrylic materials. It is strongly advised to fabricate the design from bio-inert materials such as acrylic or polycarbonate, which have proven to be effective alternatives. Further testing with PLA is pending at this time. Feel free to adjust the dimensions according to your specific requirements.