Autostainer for Biological Specimens 3D Printed USB Powered

thingiverse

This project utilizes a 3D printer, inexpensive electronic parts, a USB-powered autostainer, stepper motors, motor driver boards, an Arduino UNO microcontroller, LCD Shield, and custom ABS plastic components to create a fully programmable staining platform. The microcontroller is programmed via an LCD Shield interface to execute a 5-step staining protocol, with future developments including low-cost autostainers for high schools and additional modules such as auto-immunostainers or Thermofoil heated Real Time PCR platforms. Powered by USB, the system's potential for field research using solar panels is also considered. Leveraging 3D printed parts and frames allows rapid prototyping of low-cost automated laboratory equipment for educational uses and field research. Diff-Quik stain is used to test the system, with a method involving fixing and staining air-dried smears on slides. The Up!3D printer was used to print 3D parts for the prototype, with ABS plastic filament from Octave.com. The Arduino UNO microcontroller was chosen due to its digital inputs and outputs, allowing for system expansion in future developments. Bipolar stepper motors wired to Sparkfun motor drivers enable their control by the Arduino microcontroller for adequate force using USB power. The entire system is designed to be low-cost and modular.