$850.00PLAT 96-well liquid spreader

myminifactory

Application: - Slash antibody costs by up to 10 times - Conduct IF staining with 5-20 microliters of antibody/blocking solution per well - Conduct FISH with 20 microliter volumes - Allows for whole plate staining on glass-bottomed plates Parameters: Material: PLA Operating temperature: Autoclavable: No Clean using 70% and 100% ethanol. Distance between well centers: 9 mm Design basis: Corning Costar 96-well plate Protocol: Minor modifications to staining protocols may be necessary. During testing, we established that the columns retain heat after FISH incubation for 10 minutes at 80 degrees Celsius. The plate should be immediately cooled at 4 degrees Celsius to prevent slow reannealing of DNA and to improve signal-to-noise ratio. More stringent post-FISH wash with lower SSC buffer concentration should be performed to reduce cytoplasmic background staining. Modifications: Users are not advised to modify the columns to ensure that air is not trapped between the column and well.