$850.00PLAT 24-well liquid spreader

$850.00PLAT 24-well liquid spreader

myminifactory

Application: - Slash antibody costs by up to 10 times - Perform IF staining with 5-20 microliters of antibody/blocking solution per well - Conduct FISH with 20 microliter volumes - Enables whole plate staining in glass-bottomed plates Parameters: Material: PLA Operating temperature: Autoclavable: No Clean using 70% and 100% ethanol. Distance between well centers: 19 mm Design basis: Thermo Scientific Biolite 24-well multidish 930186 Protocol: Minor adjustments to staining protocols might be necessary. During testing, we established that the columns retain heat after FISH incubation for 10 minutes at 80 degrees Celsius. The plate should immediately cool down at 4 degrees Celsius to prevent slow reannealing of DNA and to improve signal-to-noise ratio. More stringent post-FISH wash with lower SSC buffer concentration should be performed to reduce cytoplasmic background staining. Modifications: Users may attach glass coverslips or aluminum foil to the base of each column to create a flatter surface and promote ease of cleaning.

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