$850.00PLAT 12-well liquid spreader

myminifactory

Application: - Slash antibody costs by up to 10 times - Conduct IF staining with 5-20 microliters of antibody/blocking solution per well - Perform FISH with 20 microliter volumes - Enable whole plate staining on glass-bottomed plates Parameters: Material: Polylactic acid (PLA) Operating temperature: Autoclavable: No Clean using 70% and 100% ethanol. Distance between well centers: 26 millimeters Design basis: Greiner Bio-one Cellstar 12-well plate Protocol: Minor modifications to staining protocols may be required. During testing, we found that the columns retain heat after FISH incubation for 10 minutes at 80 degrees Celsius. The plate should immediately cool down at 4 degrees Celsius to prevent slow reannealing of DNA and improve signal to noise ratio. Perform more stringent post-FISH wash with lower SSC buffer concentration to reduce cytoplasmic background staining. Modifications: Users can attach glass coverslips or aluminum foil to the base of each column to create a flatter surface and promote ease of cleaning.